《植物生理学报》 2010, 46(1): 17-23

大豆GmCOL8 基因的克隆及表达分析

马锦花¹, 张清哲¹, 陈新建^1,*, 傅永福^2,*

1 河南农业大学生命科学学院, 郑州 450002; 2 中国农业科学院作物科学研究所, 农作物基因资源与基因改良国家重大科学 工程, 北京 100081

通信作者：陈新建；E-mail: xinjian@371.net, fuyf@caas.net.cn；Tel: 0371-63558722

摘　要：

从大豆品种 ‘ 垦农18’ 中克隆了一个 CONSTANS-like 基因, 命名为GmCOL8。进化树分析表明它属于CONSTANS-like 亚家族 1 的成员。mRNA 表达分析显示, GmCOL8 在短日下具有明显的生物钟节律性表达特性, 其表达高峰出现在凌晨。 GmCOL8 主要在复叶中表达, 表达高峰出现在开花时期。

关键词：大豆; CONSTANS-like; 生物钟节律; 生物信息学

收稿：2009-09-25   修定：2009-12-29

资助：国家支撑计划(2007BAD59B02)、转基因专项(2008ZX08009-001、2008ZX08004-005、2008ZX08010-004)、“863” 项目(2006AA10Z107、2006AA10A111 、2007AA10Z119)和“973 ” 项目(2004CB117206)。

Cloning and Expression Analysis of GmCOL8 Gene in Soybean (Glycine max L.)

MA Jin-Hua¹, ZHANG Qing-Zhe¹, CHEN Xin-Jian^1,*, FU Yong-Fu^2,*

¹College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China; ²National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Corresponding author: CHEN Xin-Jian; E-mail: xinjian@371.net, fuyf@caas.net.cn; Tel: 0371-63558722

Abstract:

A CONSTANS-like gene, named GmCOL8, was cloned from soybean (Glycine max) cultivar ‘Kennong 18’ in the study. The analysis of phylogenetic tree showed that GmCOL8 was a member of soybean CONSTANS-like subfamily group 1. The mRNA expression pattern revealed that GmCOL8 had rhythmicity of circadian clock under the condition of short day, and reached a peak at dawn. It expressed mainly in trifoliolates, and the maximum level appeared at the stage of flowering.

Key words: Glycine max; CONSTANS-like; circadian clock; bioinformatics